An OspA antigen-capture enzyme-linked immunosorbent assay for detecting North American isolates of Borrelia burgdorferi in larval and nymphal Ixodes dammini

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An OspA antigen-capture enzyme-linked immunosorbent assay for detecting North American isolates of Borrelia burgdorferi in larval and nymphal Ixodes dammini.

An antigen-capture enzyme-linked immunosorbent assay (ELISA) was developed for detecting North American isolates of Borrelia burgdorferi in larval, nymphal, and adult ticks. The assay uses an anti-OspA monoclonal antibody (H5332) for antigen capture and biotin-labelled polyclonal sera with streptavidinhorseradish peroxidase for signal generation. The assay recognized 15 of 15 North American B. ...

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ژورنال

عنوان ژورنال: Journal of Clinical Microbiology

سال: 1993

ISSN: 0095-1137,1098-660X

DOI: 10.1128/jcm.31.2.272-278.1993